Identification of a gene encoding a homocitrate synthase isoenzyme of Saccharomyces cerevisiae
Ramos, F × Verhasselt, P Feller, A Peeters, P Wach, A Dubois, E Volckaert, Guido #
John wiley & sons ltd
Yeast vol:12 issue:13 pages:1315-1320
In Saccharomyces cerevisiae, most of the LYS structural genes have been identified except the genes encoding homocitrate synthase and alpha-aminoadipate aminotransferase. Expression of several LYS genes responds to an induction mechanism mediated by the product of LYS14 and an intermediate of the pathway, alpha-aminoadipate semialdehyde (alpha AASA) as an inducer. This activation is modulated by the presence of lysine in the growth medium leading to an apparent repression. Since the first enzyme of the pathway, homocitrate synthase, is feedback inhibited by lysine, it could be a major element in the control of alpha AASA supply.