Title: Generation of chicken growth hormone-binding proteins by proteolysis
Authors: Vleurick, L ×
Kuhn, ER
Decuypere, Eddy
Burnside, J
Pezet, A
Edery, M #
Issue Date: Feb-1999
Publisher: Academic press inc
Series Title: General and Comparative Endocrinology vol:113 issue:2 pages:283-289
Abstract: A soluble protein that specifically bound growth hormone (GH) was characterized in culture medium of a COS-7 cell line transfected with the cDNA of the full-length chicken GH receptor (cGHR). Incubation of culture medium with I-125-labeled human GH resulted in the formation of a single specific complex with high affinity (K-D = 0.36 nM) and apparent molecular weight of 75 kDa. The production of large quantities of GH-binding protein (GHBP) amounting to, per hour, 23% of the cell's GHR, points to the importance of partial proteolysis for GHR turnover. Considerable amounts of GHBP were also detected in a cytosolic fraction. These results strongly suggest that in chicken, as in rabbit and monkey, the GHBP is generated, at least partially, by proteolytic cleavage of the membrane-anchored GHR. (C) 1999 Academic Press.
ISSN: 0016-6480
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Livestock-Nutrition-Quality (-)
× corresponding author
# (joint) last author

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