Two experiments were conducted to investigate the effect of RH (35, 60, and 85%) on thermoregulation of broiler chickens at high (35 degrees C) and mild (30 degrees C) temperatures at the age of 4 wk. The effects of humidity on rectal temperature (RT) and plumage temperature at back (PBAT) and skin temperature at breast (SBRT) were determined at 1, 4, 8, 16, and 24 h after exposure. The RT, PBAT, and SBRT were all significantly increased by high temperature (35 degrees C). Humidity had a significant influence on RT at 35 degrees C but not at 30 degrees C. The peripheral temperatures (PBAT and SBRT) were significantly affected by humidity but responded differently at high (35 degrees C) compared with mild temperature (30 degrees C). In conclusion, high humidity above 60% impaired the heat transmission from body core to the periphery at 35 degrees C but facilitated it at 30 degrees C in 4-wk-old broiler chickens. The effect of humidity on nonevaporative heat loss was depended on air temperature, as nonevaporative heat loss was suppressed by high humidity (>60% RH) at high temperature but enhanced at the mild temperature. The effect of humidity on the relationship between peripheral and core temperature depends on ambient temperature as well as on the age of the broiler chicken. The disturbance of thermal balance could not be determined only by changes in RT or peripheral temperature at a single time point but could be determined by mean body temperature within a certain time frame.