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Title: Influence of arginine, ornithine, DFMO and polyamines on division of the generative nucleus in cultured pollen tubes of Aechmea fasciata (Bromeliaceae)
Authors: Vervaeke, Ine ×
Stichelbout, L
Londers, Elsje
Deroose, R
De Proft, MP #
Issue Date: Apr-2005
Publisher: Springer
Series Title: Plant cell tissue and organ culture vol:81 issue:1 pages:77-82
Abstract: During in vitro pollen tube growth of Aechmea fasciata the second pollen mitosis (PM 11) that produces two sperm cells was influenced by exogenous amino acids. Arginine (Arg) as single amino acid was the limiting factor for the second mitosis of the generative nucleus and thus the formation of sperm cells in cultured pollen tubes of A. fasciata. The involvement of Arg was probably related to protein synthesis. The need for Arg was not related to polyamine (PA) biosynthesis, since PA added to the germination medium were unfavourable for sperm cell production. Both ornithine (Orn) and difluoromethylornithine (DFMO) inhibited the second mitosis in cultured pollen tubes of A. fasciata. The addition of Arg during the first 2 h of pollen germination was necessary to establish the division of the generative nucleus 6 h later.
URI: 
ISSN: 0167-6857
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
× corresponding author
# (joint) last author

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