The European Union (EU) has set ammonia emission restrictions for the different member states. To comply with the new regulations, the EU wants new techniques such as adapted feeding strategies and low emission building techniques to reduce ammonia emissions from livestock buildings. However, such ammonia restriction measures are only useful when their efficiency can be measured in the field. Current reliable measuring techniques are mainly based on continuous measurements over a long period (up to 200 days), and this requires expensive equipment. This makes them unsuitable for permanent evaluation on a large number of buildings. The main objective of the study presented here was the development of a procedure to determine NH3 emissions from livestock buildings in the field, based on a limited number of measuring days. This method allows several buildings to be evaluated 'simultaneously' with the same measuring installation.