Title: Effectiveness of three micropropagation techniques to dissociate cytochimeras in Musa spp
Authors: Roux, N ×
Dolezel, J
Swennen, Rony
Zapata-Arias, FJ #
Issue Date: 2001
Publisher: Kluwer academic publ
Series Title: Plant cell tissue and organ culture vol:66 issue:3 pages:189-197
Abstract: In vitro mutagenesis of multicellular meristems of Musa spp. leads to a high degree of chimerism. Repeated vegetative propagation must be carried out to dissociate chimeras but the minimum number of cycles required is unknown. In general, mutated cells are difficult to monitor but mutations which result in a change in genome number are an exception. We simulated this case by colchicine treatment, followed by flow cytometric analysis. Colchicine treatment induced ploidy chimerism (mixoploidy), and chimera dissociation was assessed using three different propagation systems (shoot-tip culture technique - ST, multi-apexing culture technique - MA and corm slice culture technique - CS). The average percentage of cytochimeras was reduced from 100% to 36% after three subcultures using shoot-tip culture, from 100% to 24% when propagating by the corm slice culture technique and from 100% to 8% after the same number of subcultures using the multi-apexing technique. All propagation systems failed to eliminate chimerism completely. Factors that may influence chimera dissociation in vitro are discussed.
ISSN: 0167-6857
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
× corresponding author
# (joint) last author

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