Title: A comparision of four methods for cryopreservation of meristems in banana (Musa spp.)
Authors: Agrawal, A ×
Swennen, Rony
Panis, Bart #
Issue Date: Mar-2004
Publisher: Cryo letters
Series Title: Cryoletters vol:25 issue:2 pages:101-110
Abstract: To establish an improved protocol for the cryopreservation of banana (Musa spp.), a fast-fteeze/fast-thaw method was applied and compared to three existing cryogenic procedures, namely, simple freezing, vitrification of proliferating meristems and vitrification of individual meristems. The average post-thaw shoot regeneration of the fast freeze/fast thaw protocol was 51.8% and was superior to the existing protocols which were 12.5%, 39.2% and 42.8%, respectively. Other factors such as time and ease of operation were considered for each method. It was found that the fast freeze/fast thaw technique was the best amongst all the four methods tested for the cryopreservation of in vitro banana meristems in the genotype Robusta (AAA).
ISSN: 0143-2044
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
× corresponding author
# (joint) last author

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