Title: Priming of piglets against enterotoxigenic E-coli F4 fimbriae by immunisation with FAEG DNA
Authors: Verfaillie, T ×
Melkebeek, V
Snoek, V
Douterlungne, S
Cox, E
Verdonck, F
Vanrompay, Daisy
Goddeeris, Bruno
Cox, E #
Issue Date: Apr-2004
Publisher: Elsevier sci ltd
Series Title: Vaccine vol:22 issue:13-14 pages:1640-1647
Abstract: Early vaccination is necessary to protect pigs against postweaning diarrhoea caused by enterotoxigenic Escherichia coli (ETEC). However, at preset no commercial vaccine allows successful vaccination. This is partly due to the presence of maternally derived antibodies. Since DNA vaccines are suggested to be superior to protein vaccines in young animals with maternal antibodies, we determined whether the fimbrial adhesin (FaeG) of F4ac(+) ETEC could be used as a plasmid DNA vaccine to prime piglets in a heterologous prime-boost approach. Hereto, pcDNA1/faeG19 was constructed and expression of rFaeG in Cos-7 cells was demonstrated. Thereafter, pigs were immunised (days 0, 21 and 42) intramuscularly by injection or intradermally by gene gun and humoral and cellular immune responses were analysed. Even though responses were low, results demonstrated that intramuscular injection was superior to gene gun delivery for priming the humoral immune response since higher antibody titres were raised, whereas gene gun delivery better induced a cellular response, evaluated by a lymphocyte proliferation assay. Effective priming of the humoral immune response was evidenced by high IgG titres 1 week after a protein boost with purified F4. The low responses to the pcDNA1/faeG19 DNA vaccination suggest that delivery of the DNA and/or the expression of the faeG gene should be improved. (C) 2004 Elsevier Ltd. All rights reserved.
ISSN: 0264-410X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Division of Livestock-Nutrition-Quality (-)
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
Verfaillie 2004 Vaccine.pdf Published 184KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science