Author:

Keywords:

somatic embryogenesis, micro-propagation, somaclonal variation, cell suspension, yield characteristics, solanum-tuberosum, cultures, transformation, stability, Science & Technology, Life Sciences & Biomedicine, Biology, Physiology, Life Sciences & Biomedicine - Other Topics, SOLANUM-TUBEROSUM, CULTURES, TRANSFORMATION, STABILITY, 0699 Other Biological Sciences, 1116 Medical Physiology, Plant Biology & Botany, 3003 Animal production, 3199 Other biological sciences

Abstract:

This study describes the in-field behavior of bananas (Musa AA sp.) obtained after regeneration of cryopreserved embryogenic cell suspensions. Observations were focused on the classical vegetal development descriptors. We observed no significant differences between the cryopreserved-derived plants and the control plants with respect to the plant height and circumference, the number of leaves, the number of fruits, the fruit length, the fruit diameter and weight, the bunch weight and the date of harvest. During the first culture cycle, 2 out of 11 descriptors analyzed were however found to be different between the control and the cryopreserved suspensions derived plants. These were the number of nodal clusters of the inflorescence (usually called "hands") and the date of flowering. These differences were, however, quite minor as the two cases together amounted to only 2 % of the control value. During the second cycle of culture, no significant difference between the two groups of plants was found whatever the parameter analysed. These results suggest that, with the experimental conditions of the study, there is no difference at the agronomic level between plants produced from cryopreserved embryogenic cell suspensions and control plants.