Title: Identification of commercial chicory cultivars for hydroponic forcing and their phenetic relationships revealed by random amplified polymorphic DNAs and amplified fragment length polymorphisms
Authors: Van Stallen, N ×
Noten, V
Demeulemeester, M
De Proft, Maurice #
Issue Date: Jun-2000
Publisher: Blackwell wissenschafts-verlag gmbh
Series Title: Plant breeding vol:119 issue:3 pages:265-270
Abstract: One-hundred and twenty-four amplified fragment length polymorphism (AFLP) and 49 random amplified polymorphic DNA (RAPD) markers have been used to distinguish between 20 and 23 commercial chicory cultivars, respectively. These were all Cichorium intybus var. foliosum F-1 hybrids, currently used in hydroponic forcing. Five-hundred and twenty RAPD primers (OPERON) were tested, of which 156 resulted in reproducible patterns and 26 yielded polymorphisms. Two-hundred and fifty-six AFLP primer-combinations were tested and six combinations were selected for identification purposes. Similarity indices were measured and clustering has been done using pairwise comparison. Both types of marker provide similar conclusions. Two major clusters are formed, representing late and early cultivars. All cultivars were identified using 10 informative RAPD primers or three AFLP primer combinations. A low degree of polymorphism was detected between some early cultivars, suggesting a narrow genetic base in their breeding strategy.
ISSN: 0179-9541
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
× corresponding author
# (joint) last author

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