Title: Chemotactic movement and attachment of Agrobacterium tumefaciens to banana cells and tissues
Authors: Hernandez, JBP * ×
Pérez Hernández, J.B * ×
Remy, Serge
Sauco, VG
Swennen, Rony
Sagi, Laszlo #
Issue Date: Aug-1999
Publisher: Gustav fischer verlag
Series Title: Journal of plant physiology vol:155 issue:2 pages:245-250
Abstract: For the elaboration of efficient Agrobacterium-mediated transformation of banana the initial steps in the plant-bacterium interaction were investigated. Chemotaxis of agrobacteria towards tissues was studied using a swarm agar plate system. Excised leaf, rhizome, root and in vitro proliferating tissues from different banana landraces were found to be able to elicit a positive chemotactic reaction of A. tumefaciens that could be enhanced by extensive wounding. Agrobacterium attachment was examined by UV fluorescence microscopy and scanning electron microscopy, which demonstrated the presence of bacteria individually bound or massively attached to the surface of single banana cells and tissues. It can be concluded that at least during the two early steps of interaction A. tumefaciens appears to be compatible with banana, indicating the potential for genetic transformation.
ISSN: 0176-1617
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
* (joint) first author
× corresponding author
# (joint) last author

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