Title: The structural proteome of Pseudomonas aeruginosa bacteriophage phi KMV
Authors: Lavigne, Rob ×
Noben, JP
Hertveldt, Kirsten
Ceyssens, Pieter-Jan
Briers, Yves
Dumont, D
Roucourt, Bart
Krylov, VN
Mesyanzhinov, VV
Robben, Johan
Volckaert, Guido #
Issue Date: Feb-2006
Publisher: Society for General Microbiology
Series Title: Microbiology-SGM vol:152 pages:529-534
Abstract: The structural proteome of phi KMV, a lytic bacteriophage infecting Pseudomonas aeruginosa, was analysed using two approaches. In one approach, structural proteins of the phage were fractionated by SDS-PAGE for identification by liquid chromatography-mass spectrometry (LC-MS). In a second approach, a whole-phage shotgun analysis (WSA) was applied. WSA uses trypsin digestion of whole phage particles, followed by reversed-phase HPLC and gas-phase fractionation of the complex peptide mixture prior to MS. The results yield a comprehensive view of structure-related proteins in phi KMV and suggest subtle structural differences from phage T7.
ISSN: 1350-0872
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Biochemistry, Molecular and Structural Biology Section
Laboratory of Glycobiology and Developmental Genetics (-)
× corresponding author
# (joint) last author

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