Title: SuperSAGE combined with PCR walking allows global gene expression profiling of banana (Musa acuminata), a non-model organism
Authors: Coemans, Bert ×
Matsumura, H
Terauchi, R
Remy, Serge
Swennen, Rony
Sagi, Laszlo #
Issue Date: Oct-2005
Publisher: Springer
Series Title: Theoretical and applied genetics vol:111 issue:6 pages:1118-1126
Abstract: Super-serial analysis of gene expression (SuperSAGE) was used to characterize, for the first time, the global gene expression pattern in banana (Musa acuminata). A total of 10,196 tags were generated from leaf tissue, representing 5,292 expressed genes. Forty-nine tags of the top 100 most abundantly expressed transcripts were annotated by homology to cDNA or EST sequences. Typically for leaf tissue, analysis of the transcript profiles showed that the majority of the abundant transcripts are involved in energy production, mainly photosynthesis. However, the most abundant tag was derived from a type 3 metallothionein transcript, which accounted for nearly 3% of total transcripts analysed. Furthermore, the 26-bp long SuperSAGE tags were applied in 3'-rapid amplification of cDNA ends (3'RACE) for the identification of unknown tags. In combination with thermal asymmetric interlaced PCR (TAIL-PCR), this allowed the recovery of a full gene sequence of a novel NADPH:protochlorophyllide oxidoreductase, the key enzyme in chlorophyll biosynthesis. SuperSAGE in conjunction with 3'RACE and TAIL-PCR will be a powerful tool for transcriptomics of non-model, but otherwise important organisms.
ISSN: 0040-5752
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science