Title: 3,3 ',5-triiodothyronine (T-3) uptake and expression of thyroid hormone receptors during the adaptation to hypothyroidism of the brain of chicken
Authors: Gereben, B ×
Kollar, A
Bartha, T
Buys, Nadine
Decuypere, Eddy
Rudas, P #
Issue Date: 1998
Publisher: Akademiai kiado
Series Title: Acta veterinaria hungarica vol:46 issue:4 pages:473-485
Abstract: Thyroid hormone action in the brain is strictly regulated, since these hormones play a crucial role in the development and physiological functioning of the central nervous system. Hormone kinetics and molecular events at the nuclear receptor level during the adaptation of the brain of chicken to hypothyroidism were simultaneously investigated. Data obtained by Oldendorff's 'single-pass' technique showed a significantly higher labelled 3,3',5-triiodothyronine (I-125-T-3) uptake into the brain of surgically thyroidectomized (TX) 2-week-old broilers after 1 week of the surgery in comparison to sham-operated (SH) and T-3 supplemented (TX + T-3) controls in the 10th second after the bolus injection. Telencephalons showed the highest, while cerebellum the lowest uptake intensity in all groups. In a similar arrangement of experiments the expression of the TR alpha- and TR beta nuclear thyroid receptors in the telencephalon of TX and control chickens was investigated by a semiquantitative RT-PCR study in the following way: cDNAs were standardised using a quantitative RT-PCR-based approach for beta-actin, then amplified for thyroid receptors. The level of both the TR alpha and TR beta coding mRNA was elevated in hypothyroidism. In conclusion, the presented hormone kinetics and TR expression data provide further details of the cellular and molecular events occurring during the adaptation to hypothyroidism of the brain of chicken.
ISSN: 0236-6290
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Division of Livestock-Nutrition-Quality (-)
× corresponding author
# (joint) last author

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