Fundamental and applied nematology vol:19 issue:5 pages:407-414
Transmission electron microscopy is used to describe the intoxication in Caenorhabditis elegans, feeding on toxic spore/crystals of Bacillus thuringiensis. The toxin acts directly against the intestine, first by affecting the anteriormost ring of four intestinal cells. Over a period of 12 hours, these cells lose much of their volume, the microvilli regress slowly, several cell organelles undergo dramatic change and are ultimately destroyed. No rupture of the apical intestinal cell membrane is observed. Non-intestinal tissues seem unaffected. This study indicates considerable ultrastructural differences in the mode of action between the nematicidal toxin and the insecticidal crystal toxins from B. thuringiensis.