Title: Agrobacterium rhizogenes-mediated transformation of Sesbania rostrata
Authors: Van de Velde, W ×
Mergeay, Joachim
Holsters, M
Goormachtig, S #
Issue Date: Dec-2003
Publisher: Elsevier sci ireland ltd
Series Title: Plant science vol:165 issue:6 pages:1281-1288
Abstract: In order to study the function of nodulin genes and the activity of their promoters, the Agrobacterium rhizogenes transformation strategy was adopted for Sesbania rostrata. Two protocols were selected to generate S. rostrata transgenic roots that can be nodulated efficiently after application of Azorhizobium caulinodans, ORS571. Cotransformation frequencies of 22 and 72% were obtained with the first and second protocol, respectively. The transgenic root nodules showed no apparent differences when compared with the wild-type root nodules. The screening procedure for cotransformation of binary T-DNA in transgenic roots and root nodules was optimized by using an enhanced green-fluorescent protein construct. The A. rhizogenes transformation system was used to analyze the 35S promoter-driven expression of reporter genes in developing root nodules. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
ISSN: 0168-9452
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory for Aquatic Ecology and Evolutionary Biology (-)
× corresponding author
# (joint) last author

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