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Title: Transgenic expression in tobacco of a poly-proctolin construct leading to production of the bioactive peptide
Authors: Rao, R ×
Breuer, Michael
Tortiglione, C
Malva, C
Baggerman, Geert
Corrado, G
De Loof, Arnold
Pennacchio, F #
Issue Date: Sep-2004
Publisher: Kluwer academic publ
Series Title: Biotechnology letters vol:26 issue:18 pages:1413-1420
Abstract: Short peptides can be expressed in plants using synthetic genes encoding multiple copies of the peptide spaced by dibasic endoproteolytic cleavage sites. A synthetic gene encoding an array of repeated copies of proctolin, a very well characterized insect myotropic peptide, spaced by Arg residues, was synthesized and expressed in tobacco plants. The successful production of bioactive proctolin from the precursor in transgenic plants was demonstrated by immunoblot, HPLC, mass spectrometry and a bioassay based on the contraction of isolated cockroach hindgut. These results suggest that in planta, as in animals and yeasts, endopeptidases of the serine proteases family may be involved in precursor processing.
ISSN: 0141-5492
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Department of Biology - miscellaneous
Microbial and Molecular Systems - miscellaneous
× corresponding author
# (joint) last author

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