Title: Search for phase specific genes in the brain of desert locust, Schistocerca gregaria (Orthoptera : Acrididae) by differential display polymerase chain reaction
Authors: Rahman, MM ×
Vandingenen, Anick
Begum, M
Breuer, Michael
De Loof, Arnold
Huybrechts, Roger #
Issue Date: Jun-2003
Publisher: Elsevier science inc
Series Title: Comparative biochemistry and physiology a-molecular & integrative physiology vol:135 issue:2 pages:221-228
Abstract: Differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) in combination with semi-quantitative RT-PCR was used to compare differences in gene expression between the solitary and gregarious phase of Schistocerca gregaria. Twenty-six primer combinations were used, which produced 8 differential bands. Two out of the 8 differentials, one typical for the solitary and one for the gregarious phase, were further analyzed by semi-quantitative RT-PCR. The expression level of the solitary phase specific gene (SSG) was 2 times higher in solitary animals as compared to gregarious ones, while the gregarious specific gene (GSG) gave a 4-fold higher expression level in gregarious animals than in solitaries. Sequence analysis demonstrated that SSG does not belong to a known gene family, while the GSG belongs to the SPARC protein family. (C) 2003 Elsevier Science Inc. All rights reserved.
ISSN: 1095-6433
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Department of Biology - miscellaneous
× corresponding author
# (joint) last author

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