Title: In vitro synthesis of fructofuranosyl-only oligosaccharides from inulin and fructose by purified chicory root fructan:fructan fructosyl transferase
Authors: Van den Ende, Wim ×
DeRoover, J
Van Laere, André #
Issue Date: Jun-1996
Publisher: Munksgaard int publ ltd
Series Title: Physiologia plantarum vol:97 issue:2 pages:346-352
Abstract: Besides the non-reducing inulin series of gluco-fructo oligosaccharides which are common in Asteraceae, two series of reducing fructofuranosyl-only oligosaccharides occur in overwintering, forced, and sprouting chicory roots. Such inulo-n-oses could br produced by the action of an endo-inulinase on gluco-fructo oligosaccharides. We show here that similar series of inulo-n-oses as found in vivo can be produced by incubating fructose and inulin with a purified fructan:fructan fructosyl transferase (FFT; EC from chicory roots (Cichorium intybus L.). Therefore fructose (but not glucose) can act as an acceptor for FFT. Since the inulo-n-oses only appear in vivo once the endogenous fructose concentration increases, we conclude that they are not the result of a putative endo-inulinase but of FFT activity.
ISSN: 0031-9317
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
× corresponding author
# (joint) last author

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