Title: Neuropeptidomics of the grey flesh fly, Neobellieria bullata
Authors: Verleyen, Peter
Huybrechts, Jurgen
Sas, Filip
Clynen, Elke
Baggerman, Geert
De Loof, Arnold
Schoofs, Liliane # ×
Issue Date: Apr-2004
Publisher: Academic Press
Series Title: Biochemical and Biophysical Research Communications vol:316 issue:3 pages:763-770
Abstract: A peptidomics approach was applied to determine the peptides in the larval central nervous system of the grey flesh fly, Neo-bellieria bullata. Fractions obtained by high performance liquid chromatography were analysed by MALDI-TOF and ESI-Q-TOF mass spectrometry. This provided biochemical evidence for the presence of 18 neuropeptides, 11 of which were novel Neobellieria peptides. Most prominently present were the FMRFamide-related peptides: 7 FNIRFamides, 1 FIRFamide, and Neb-myosuppressin. The three putative capa-gene products Neb-pyrokinin and the periviscerokinins Neb-PVK-1 and -2 were detected, as well as another pyrokinin. This Neb-PK-2 was also present in the ring gland along with corazonin, Neb-myosuppressin, and Neb-AKH-GK, an intermediate processing product of the adipokinetic hormone. Furthermore, the central nervous system contained Neb-LFamide, proctolin, and FDFHTVamide, designated as Neb-TVamide. With this study, we considerably increased our knowledge of the neuropeptidome of the pest fly N. bullata, which is an important insect model for physiological research. (C) 2004 Elsevier Inc. All rights reserved.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Department of Biology - miscellaneous
Microbial and Molecular Systems - miscellaneous
× corresponding author
# (joint) last author

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