Isolation and multiplex analysis of six polymorphic microsatellites in the Antarctic notothenioid fish, Trematomus newnesi

Van Houdt, JKJ; Hellemans, Bart; Van de Putte, Anton; Koubbi, P; Volckaert, Filip

doi:10.1111/j.1471-8286.2005.01174.x

Isolation and multiplex analysis of six polymorphic microsatellites in the Antarctic notothenioid fish, Trematomus newnesi

Author:

Van Houdt, JKJ

Hellemans, Bart ; Van de Putte, Anton ; Koubbi, P ; Volckaert, Filip

Keywords:

antarctic, microsatellite, nototheniidae, nuclear marker, trematomids, Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Ecology, Evolutionary Biology, Environmental Sciences & Ecology, Antarctic, Nototheniidae, Trematomids, 31 Biological sciences

Abstract:

Six polymorphic microsatellites were isolated from the dusky notothen, Trematomus newnesi (Boulenger 1902), using a microsatellite enrichment protocol and selective hybridization with di- tetra- and penta-repeat probes. The loci were screened in 48 individuals captured in the Southern Ocean (coastal zone of Terre-Adelie), revealing eight to 22 alleles per locus and an observed heterozygosity ranging from 0.70 to 0.92. These microsatellite markers provide a tool to study the relationship between the various morphs observed in this species and can be used for population genetic analysis and biodiversity studies.