Title: Functional and comparative analysis of two distinct ecdysteroid-responsive gene expression constructs in Drosophila S2 cells
Authors: Poels, Jeroen ×
Martinez, A
Suner, MM
De Loof, Arnold
Dunbar, SJ
Vanden Broeck, Jozef #
Issue Date: May-2004
Publisher: Pergamon-elsevier science ltd
Series Title: Insect biochemistry and molecular biology vol:34 issue:5 pages:451-458
Abstract: Inducible expression systems have proven to be of major interest when analysing the function of specific genes or when expressing cytotoxic proteins. In an effort to develop inducible switches allowing for flexible fine-tuning of gene expression levels in insect cells, we have compared the induction capacities of two Drosophila minimal promoters when linked to four consecutive ecdysone response elements. These minimal promoters, either containing a TATA-box or a downstream promoter element, drove the expression of a luciferase reporter gene. Potent induction capacities were observed with the insect moulting hormone, 20-hydroxyecdysone, and with ponasterone A, a plant ecdysteroid. The developed inducible switches further expand the repertoire of molecular tools for functional expression of proteins of interest in insect cells. In addition, the combination of an ecdysone switch with promoters that possess different structural elements can provide novel insights into ecdysteroid-induced transcription in an insect cell line. (C) 2004 Elsevier Ltd. All rights reserved.
ISSN: 0965-1748
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Department of Biology - miscellaneous
× corresponding author
# (joint) last author

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