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Title: Extraction of high-quality genomic DNA from latex-containing plants
Authors: Michiels, An ×
Van den Ende, Wim
Tucker, M
Van Riet, Liesbet
Van Laere, André #
Issue Date: Apr-2003
Publisher: Academic press inc elsevier science
Series Title: Analytical biochemistry vol:315 issue:1 pages:85-89
Abstract: The isolation of intact, high-molecular-mass genomic DNA is essential for many molecular biology applications including long PCR, endonuclease restriction digestion, Southern blot analysis, and genomic library construction. Many protocols are available for the extraction of DNA from plant material. However, for latex-containing Asteraceae (Cichorioideae) species, standard protocols and commercially available kits do not produce efficient yields of high-quality amplifiable DNA. A cetyltrimethylammonium bromide protocol has been optimized for isolation of genomic DNA from latex-containing plants. Key steps in the modified protocol are the use of etiolated leaf tissue for extraction and an overnight 25 degreesC isopropanol precipitation step. The purified DNA has excellent spectral qualities, is efficiently digested by restriction endonucleases, and is suitable for long-fragment PCR amplification. (C) 2003 Elsevier Science (USA). All rights reserved.
URI: 
ISSN: 0003-2697
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
× corresponding author
# (joint) last author

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