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Title: A radioimmunoassay for African catfish growth hormone: Validation and effects of substances modulating the release of growth hormone
Authors: Lescroart, Olivier ×
Roelants, Ivo
Mikolajczyk, T
Bosma, PT
Schulz, RW
Kuhn, ER
Ollevier, Frans #
Issue Date: Nov-1996
Publisher: Academic press inc jnl-comp subscriptions
Series Title: General and Comparative Endocrinology vol:104 issue:2 pages:147-155
Abstract: A highly sensitive radioimmunoassay has been developed for measuring plasma growth hormone (GH) concentrations in the African catfish (Clarias gariepinus). The lower detection limit of the assay was 0.1 ng/ml and the standard curve had an ED(50) value of 0.5 ng/ml. The validity of the assay was established and the effects of several neurotransmitters on the release of GK were examined. In vitro experiments, using a static culture system for dispersed pituitary cells, demonstrated that the GH release in African catfish was affected by growth hormone-releasing hormone and somatostatin. Single intraperitoneal injections with a dopamine agonist, apomorphine, produced significant and dose-dependent increases in plasma GH levels. Unlike carp, goldfish, and tilapia, a super-active analogue of salmon gonadotrophin-releasing hormone did not alter plasma GH levels in African catfish. (C) 1996 Academic Press, Inc.
URI: 
ISSN: 0016-6480
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Ecology, Evolution and Biodiversity Conservation Section
× corresponding author
# (joint) last author

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