Title: Cloning of the cDNA encoding Scg-SPRP, an unusual Ser-protease-related protein from vitellogenic female desert locusts (Schistocerca gregaria)
Authors: Chiou, SJ ×
Vanden Broeck, Jozef
Janssen, Ine
Borovsky, D
Vandenbussche, Frank
Simonet, Gert
De Loof, Arnold #
Issue Date: Oct-1998
Publisher: Pergamon-elsevier science ltd
Series Title: Insect biochemistry and molecular biology vol:28 issue:10 pages:801-808
Abstract: The cDNA coding for a Ser-protease-related protein (Scg-SPRP)(1) was cloned from desert locust (Schistocerca gregaria) midgut. The derived amino acid sequence consists of 260 residues and shows strong sequence similarity to insect trypsin-like molecules. It is, however, likely that Scg-SPRP is not a proteolytically active enzyme and that it plays another physiologically relevant role, since two out of three residues which are indispensable for catalytic activity of Ser-proteases are replaced. Northern analysis revealed that the Scg-SPRP gene is expressed in midgut tissue and that this expression is strongly induced in adult female locusts. Moreover, the occurrence of the transcript (1.2 kb) fluctuates during the molting cycle and during the female reproductive cycle. Juvenile hormone (JH III) dependence of transcription was investigated by chemical allatectomy (precocene I) of adult females. This resulted in inhibition of vitellogenesis and in disappearance of the Scg-SPRP transcript. Expression of Scg-SPRP in precocene-treated locusts could be reinduced by additional treatment with JH III or with 20-OH-ecdysone. (C) 1998 Elsevier Science Ltd. All rights reserved.
ISSN: 0965-1748
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
× corresponding author
# (joint) last author

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