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Title: In vitro adenine nucleotide catabolism in African catfish spermatozoa
Authors: Zietara, MS ×
Slominska, E
Rurangwa, Eugène
Ollevier, Frans
Swierczynski, J
Skorkowski, EF #
Issue Date: Aug-2004
Publisher: Elsevier science inc
Series Title: Comparative biochemistry and physiology b-biochemistry & molecular biology vol:138 issue:4 pages:385-389
Abstract: It has been shown recently that African catfish (Clarias gariepinus) spermatozoa possess relatively low ATP content and low adenylate energy charge (AEC). One of the possible explanations for this phenomenon is that the spermatozoa actively catabolize adenine nucleotides. A relatively high rate of such catabolism could then contribute to the low ATP concentration and low adenylate energy charge observed in the spermatozoa in vitro. To check this hypothesis, we investigated ATP content and adenine nucleotide catabolism in African catfish spermatozoa stored at 4degreesC in the presence of glycine as an energetic substrate. Our results indicate that the storage of African catfish sperm at 4degreesC in the presence of glycine causes time-dependent ATP depletion. In contrast to ATP, the AMP content increases significantly during the same period of sperm storage, while the ADP increases only slightly. Moreover, a significant increase of inosine and hypoxanthine content was also found. Hypoxanthine was accumulated in the storage medium, but xanthine was found neither in spermatozoa nor in the storage medium. It indicates that hypoxanthine is not converted to xanthine, probably due to lack of xanthine oxidase activity in catfish spermatozoa. Present results suggest that adenine nucleotides may be converted to hypoxanthine according to the following pathway: ATP-->ADP--> AMP (adenosine/IMP)-->inosine-->hypoxanthine. Moreover, hypoxanthine seems to be the end product of adenine nucleotide catabolism in African catfish spermatozoa. In conclusion, our results suggest that a relatively high rate of adenine nucleotide catabolism contributes to the low ATP concentration and low adenylate energy charge observed in African catfish spermatozoa in vitro. (C) 2004 Elsevier Inc. All rights reserved.
URI: 
ISSN: 1096-4959
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Ecology, Evolution and Biodiversity Conservation Section
Laboratory for Aquatic Ecology and Evolutionary Biology (-)
× corresponding author
# (joint) last author

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