Title: Partial identification of a peptide that stimulates the primary urine production of single isolated Malpighian tubules of the forest ant, Formica polyctena
Authors: Laenen, B
Verhaert, Peter
Schoofs, Liliane
Steels, P
Van Kerkhove, E # ×
Issue Date: Aug-1999
Publisher: Pergamon Press
Series Title: Journal of Insect Physiology vol:45 issue:8 pages:743-753
Abstract: A peptide was purified from a 10% trifluoroacetic acid (TFA) head/thorax extract of 300,000 ants with high performance liquid chromatography (HPLC). Fluid secretion assay of single isolated Malpighian tubules was used as a bioassay. The purity of F. polyctena diuretic peptide (FopDP) after a two step HPLC protocol was confirmed by means of mass spectrometry and revealed a molecular mass of 7514 daltons. Due to lack of material, no enzymatic digestion could be performed and the sequence of only the first 25 amino acids could be determined: VPKYENCVSEVLPAGDRQRCVKVTC. A computer search of sequence data banks did not reveal any significant similarity between FopDP and other known insect diuretic peptides.
ISSN: 0022-1910
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
× corresponding author
# (joint) last author

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