Mass spectrometric evidence for the deficiency in the dark color inducing hormone, [His(7)]-corazonin in an albino strain of Locusta migratoria as well as for its presence in solitary Schistocerca gregaria
Archives of Insect Biochemistry and Physiology vol:47 issue:3 pages:150-160
A factor present in the brain and corpus cardiacum responsible for the induction of dark colour in Locusta migratoria was recently isolated and identified from the corpora cardiaca of normally pigmented locusts. The purification of this factor, designated as [His(7)]-corazonin was monitored using an albino mutant from a laboratory colony of an Okinawa (Japan) strain. In this study, we provide unequivocal mass spectrometric evidence that the brain and the corpora cardiaca of this albino Locusta mutant are deficient in [His(7)]-corazonin. Previously, [His(7)]-corazonin was shown to be responsible for the induction of dark body colour patterns as observed in crowded locusts. Using nanoflow-liquid chromatography-mass spectrometry, we demonstrated that this dark colour-inducing hormone is, however, present in the corpora cardiaca of solitary locusts (Schistocerca gregaria). (C) 2001 Wiley-Liss, Inc.