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Title: In vivo microdialysis in the visual cortex of awake cat II: Sample analysis by microbore HPLC-electrochemical detection and capillary electrophoresis-laser-induced fluorescence detection
Authors: Qu, Y ×
Li, YM
Vandenbussche, Erik
Vandesande, Frans
Arckens, Lut #
Issue Date: Apr-2001
Publisher: Elsevier science bv
Series Title: Brain research protocols vol:7 issue:1 pages:45-51
Abstract: Sampling and monitoring release of excitatory and inhibitory amino acids in the striate cortex of mammals will provide important information for visual system research. Two microbore high performance liquid chromatography-electrochemical detection methods and a capillary electrophoresis-laser induced fluorescence detection were developed to determine the inhibitory amino acid, gamma -aminobutyric acid and the excitatory amino acids, glutamate and aspartate in microdialysates of cat striate cortex. In the liquid chromatography method, samples were derivatized using OPA-TBT. Ten microliters of derivatized product was injected onto the microbore column (100x1 mm i.d., C8) for quantitative analysis. Electrochemical detection was employed. In the capillary electrophoresis method, samples were derivatized using fluorescein isothiocyanate and separated in berate buffer within 15 min, then detected by a laser-induced fluorescence detector. (C) 2001 Elsevier Science B.V. All rights reserved.
ISSN: 1385-299X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Research Group Neurophysiology
× corresponding author
# (joint) last author

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