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Title: An effect of androgens on the length of the poly(a)-tail and alternative splicing cause size heterogeneity of the messenger ribonucleic-acids encoding cystatin-related protein
Authors: Vercaeren, I ×
Winderickx, Joris
Devos, Ann
Peeters, Benjamin
Heyns, Walter #
Issue Date: Dec-1992
Publisher: Endocrine soc
Series Title: Endocrinology vol:131 issue:6 pages:2496-2502
Abstract: The 22-kilodalton glycoprotein, expressed in the rat ventral prostate under the influence of androgens, is structurally a cystatin-related protein (CRP), as has been shown by copy DNA sequencing. In fact, two slightly different forms (CRP-1 and CRP-2) are expressed in the prostate; one of them (CRP-1) is also expressed in the exorbital lachrymal gland. In both glands, the CRP-1 messenger RNA (mRNA)s are androgen regulated. Moreover, androgens also influence the size of these mRNAs, which show marked heterogeneity (from 760-950 nucleotides). Indeed, the smaller forms are predominant in castrated animals, whereas the large forms are observed immediately after androgen induction. Hybridization with oligo(dT) followed by ribonuclease H treatment revealed that differences in length of the poly(A)-tail are responsible for this effect of androgens. Indeed, two well defined forms of CRP mRNA subsisted after removal of the poly(A)-tail by this treatment. In the less abundant shorter form (CRP-1DELTA), 123 nucleotides are deleted by alternative splicing at the junction between the third and the fourth exon. The variant mRNA encodes a truncated protein, wherein the last 27 amino acids are replaced by a hydrophobic stretch of 8 amino acids. No alternative splicing was observed for the CRP-2 mRNA.
ISSN: 0013-7227
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry Section (Medicine) (-)
Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
Clinical and Experimental Endocrinology
× corresponding author
# (joint) last author

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