Applied and Environmental Microbiology vol:61 issue:12 pages:4367-4373
The different serotyping systems, based on thermostable O antigens, reported for Vibrio anguillarum and V. ordalii were compared by quantitative agglutination, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and subsequent silver staining or Western blotting (immunoblotting) of purified lipopolysaccharide (LPS), using polyclonal rabbit antisera. The results demonstrate that 16 different serotypes within V. anguillarum (designated 01 to 016) can be distinguished, Each of these serotypes is characterized by a distinct polysaccharide banding pattern, as revealed by silver-stained gels of purified LPS, The comparative analysis allowed a complete alignment of the different serotypes for the first three serovars: 01, 02, and 03. Moreover, immunoblotting showed that strains belonging to each of these serotypes had the same LPS banding pattern independent of the origin of the strain. Serotype 02 contains different subtypes, 02a and 02b. While no differences were apparent between these subgroups in silver-stained gels, they could be separated by quantitative agglutination (titer determination) or immunoblotting. V. ordalii, the former biotype II of V. anguillarum, strongly reacts,vith anti-V. anguillarum 02a antiserum, Strains of the two species can be separated on the basis of different LPS profiles in the high-molecular-weight region of silver-stained gels of purified LPS, The silver-stained LPS profiles of the different serotypes of V. anguillarum that have been established are provided for further comparison in the future.