Title: The G protein-coupled receptor Gpr1 and the G alpha protein Gpa2 act through the cAMP-protein kinase a pathway to induce morphogenesis in candida albicans
Authors: Maidan, MM ×
De Rop, L
Serneels, Joke
Exler, S
Rupp, S
Tournu, Hélène
Thevelein, Johan
Van Dijck, Patrick #
Issue Date: Apr-2005
Publisher: Amer soc cell biology
Series Title: Molecular biology of the cell vol:16 issue:4 pages:1971-1986
Abstract: We investigated the role in cell morphogenesis and pathogenicity of the Candida albicans GPR1 gene, encoding the G protein-coupled receptor Gpr1. Deletion of C. albicans GPR1 has only minor effects in liquid hypha-inducing media but results in strong defects in the yeast-to-hypha transition on solid hypha-inducing media. Addition of cAMP, expression of a constitutively active allele of the G alpha protein Gpa2 or of the catalytic protein kinase A subunit TPKI restores the wild-type phenotype of the CaGPR1-deleted strain. Overexpression of HST7, encoding a component of the mitogen-activated protein kinase pathway, does not suppress the defect in filamentation. These results indicate that CaGpr1 functions upstream in the cAMP-protein kinase A (PKA) pathway. We also show that, in the presence of glucose, CaGpr1 is important for amino acid-induced transition from yeast to hyphal cells. Finally, as opposed to previous reports, we show that CaGpa2 acts downstream of CaGpr1 as activator of the cAMP-PKA pathway but that deletion of neither CaGpr1 nor CaGpa2 affects glucose-induced cAMP signaling. In contrast, the latter is abolished in strains lacking CaCdc25 or CaRas1, suggesting that the CaCdc25-CaRas1 rather than the CaGpr1-CaGpa2 module mediates glucose-induced cAMP signaling in C. albicans.
ISSN: 1059-1524
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Microbiology and Biotechnology Section - miscellaneous (-)
Laboratory for Molecular Cell Biology (-)
× corresponding author
# (joint) last author

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