Author:

Keywords:

candida albicans, g-protein-coupled receptor gpr1, yeast-to-hypha transition, saccharomyces-cerevisiae, pseudohyphal differentiation, nutrient sensor, morphogenesis, glucose, activation, Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Candida albicans, G-protein-coupled receptor Gpr1, SACCHAROMYCES-CEREVISIAE, PSEUDOHYPHAL DIFFERENTIATION, NUTRIENT SENSOR, MORPHOGENESIS, GLUCOSE, ACTIVATION, Amino Acids, Carbon, Culture Media, Glucose, Receptors, G-Protein-Coupled, 0601 Biochemistry and Cell Biology, 1101 Medical Biochemistry and Metabolomics, 3101 Biochemistry and cell biology

Abstract:

Yeast-to-hypha transition in Candida albicans can be induced by a wide variety of factors, including specific nutrients. We have started to investigate the mechanism by which some of these nutrients may be sensed. The G-protein-coupled receptor Gpr1 is required for yeast-to-hypha transition on various solid hypha-inducing media. Recently we have shown induction of Gpr1 internalization by specific amino acids, e.g. methionine. This suggests a possible role for methionine as a ligand of CaGpr1. Here we show that there is a big variation in methionine-induced hypha formation depending on the type of carbon source present in the medium. in addition high glucose concentrations repress hypha formation whereas a concentration of 0.1%, which mimics the glucose concentration present in the bloodstream, results in maximal hypha formation. Hence, it remains unclear whether Gpr1 senses sugars, as in Saccharomyces cerevisiae, or specific amino acids like methionine.