Title: Single-step immunoaffinity purification of the neuropeptide sericotropin using polyclonal antibodies towards the synthetic N-terminal fragment of the molecule
Authors: Kodrik, D ×
Berghman, Luc
De Loof, Arnold #
Issue Date: 1997
Publisher: Institute of Entomology, Czech Academy of Sciences
Series Title: European Journal of Entomology vol:94 issue:2 pages:307-309
Abstract: An immunoaffinity chromatography of the neuropeptide sericotropin using rabbit polyclonal antibodies towards the 16 amino acids synthetic fragment of the molecule was used for the purification of the peptide from Galleria mellonella larval brains. The method employed CNBr-activated Sepharose and the isolated IgG fraction of the corresponding antiserum. The purity of the peptide was proven by means of HPLC and immunoblotting techniques.
ISSN: 1210-5759
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Department of Biology - miscellaneous
× corresponding author
# (joint) last author

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