Title: Recombinant protein expression in insect cell systems
Authors: Huybrechts, Roger ×
Vulsteke, Veerle
Poels, Jeroen
Lauwers, Erwin
Vanden Broeck, Jozef
De Loof, Arnold #
Issue Date: Jun-1997
Publisher: Societe royale zoologique de belgique
Series Title: Belgian journal of zoology vol:127 issue:1 pages:35-45
Abstract: Since the introduction of baculovirus expression vectors, the suitability of insect cells for the expression of functionally active genes of eukaryotic origin has been exhaustively documented. Originally realization of a functional viral expression vector was laborious and depended on double homologous in vivo recombination success as well as successful purification of recombinant baculovirus by plaque assay. Using the commercial Bac to Bac(TM) expression system we confirm that transposon-assisted recombination and cloning of recombinant transfectable bacmid DNA in bacteria now allow fast productive expression of a gene of interest.
ISSN: 0777-6276
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Department of Biology - miscellaneous
Molecular Genetics Section (-)
× corresponding author
# (joint) last author

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