Comparison of different treatments for LM and SEM studies and systematic value of pollen grains in Cyperaceae
Van Wichelen, J × Camelbeke, K Chaerle, P Goetghebeur, P Huysmans, Suzy #
Scandinavian university press
Grana vol:38 issue:1 pages:50-58
The pollen grain morphology of 30 species of 27 genera from the four subfamilies of the Cyperaceae have been studied with LM and SEM. Several methods were tested in order to find the optimal treatment for the delicate Cyperaceae pollen grains. For LM, treatment with wetting agent Agepon and KOH yielded the best results, while critical point drying (CPD) after fixation with alcohol proved to be the best method for studying pollen grains of Cypfraceae with SEM. Our results show that the Mapanioideae have a pollen grain type (only one distal ulcus) clearly different from the other Cyperaceae. Representatives of the examined Sclerioideae and Caricoideae show a similar pollen grain type (mostly one distal ulcus and three lateral pores) while in the Cyperoideae different pollen grain types are found.