Title: Sequencing and characterization of trypsin modulating oostatic factor (tmof) from the ovaries of the gray fleshfly, neobellieria (sarcophaga) bullata
Authors: Bylemans, Dany ×
Borovsky, D
Hunt, Df
Shabanowitz, J
Grauwels, L
De Loof, Arnold #
Issue Date: Feb-1994
Publisher: Elsevier science bv
Series Title: Regulatory peptides vol:50 issue:1 pages:61-72
Abstract: Injection of crude extracts of late vitellogenic ovaries into staged females of the grey fleshfly Neobellieria (Sarcophaga) bullata inhibited oocyte development and biosynthesis of trypsin-like enzymes in the gut. Trypsin synthesis in N. bullata is cyclic and is correlated with egg development, which is discontinuous. A trypsin modulating oostatic factor (Neb-TMOF) was purified from 10,000 vitellogenic ovaries and sequenced by mass spectrometry. Neb-TMOF is a hexapeptide (NH2-NPTNLH-COOH). Injection of the hormone at physiological concentrations (10(-9) M), inhibited trypsin-like synthesis by the midgut of liver-fed female flies, and caused a reduction of the vitellogenin concentration in the hemolymph and of oocyte growth. The role of Neb-TMOF in controlling egg development and the physiological similarities with Aedes-TMOF are discussed.
ISSN: 0167-0115
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
Department of Biology - miscellaneous
× corresponding author
# (joint) last author

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