Title: Cloning and tissue distribution of the cyclic AMP generating peptide of the grey flesh fly Neobellieria bullata (Diptera : Sarcophagidae)
Authors: Begum, M
Macours, Nathalie
Schoofs, Liliane
Rahman, MM
De Loof, Arnold
Huybrechts, Roger # ×
Issue Date: Jul-2003
Publisher: Koninklijke Belgische Vereniging voor Dierkunde
Series Title: Belgian journal of zoology vol:133 issue:2 pages:151-156
Abstract: Originally, two forms of cyclic AMP Generating Peptide (cGP) were identified in whole body extracts of the flesh fly Neobellieria bullata. The long form, a 48-mer, stimulated cAMP production by Malpighian tubules of Manduca sexta. The short form, which had been discovered previously with an ovarian bioassay, turned out to be the 1-15 aa sequence of the 48-mer. We here report on the cloning, sequencing and expression profile of the cGP-gene in N. bullata. The full-length cDNA sequence, obtained by RT-PCR in combination with 5' and 3' RACE, encodes a single copy of the cGP precursor. No signal peptide is present in this precursor, which, compared to the mature peptide, is only extended at the N-terminus with an extra methionine residue. RT-PCR revealed that Neb-cGP is expressed in both larval and adult brain, testis and ovaries, flight muscles, Malpighian tubules, midgut, and fat body.
ISSN: 0777-6276
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Department of Biology - miscellaneous
× corresponding author
# (joint) last author

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