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Title: Activation state of protein kinase A as measured in permeabilised Saccharomyces cerevisiae cells correlates with PKA-controlled phenotypes in vivo
Authors: Portela, P ×
Van Dijck, Patrick
Thevelein, Johan
Moreno, S #
Issue Date: Mar-2003
Publisher: Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies
Series Title: FEMS Yeast Research vol:3 issue:1 pages:119-126
Abstract: Protein kinase A (PKA) activity was measured in situ in permeabilised Saccharomyces cerevisiae cells in the absence and the presence of cAMP. Four strains genetically predicted to have differential PKA-dependent phenotypes were used: a wild-type strain and a strain containing a bcy1-14 mutation (with almost constitutively active PKA), and the same strains with overexpression of the wild-type or mutant BCY1 gene, respectively. Cells were grown on galactose or glucose. The measured phenotypic characteristics were: trehalose and glycogen levels and the activity of a reporter gene under control of the NTH1 promoter. The 'endogenous' PKA activity (measured in situ in the absence of cAMP) showed the best correlation with the PKA-dependent phenotypes determined in vivo. We propose that this parameter offers a good estimate for the degree of activation of PKA in vivo. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
URI: 
ISSN: 1567-1356
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Microbiology and Biotechnology Section - miscellaneous
× corresponding author
# (joint) last author

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