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Title: The use of real-time PCR to study the expression of thyroid hormone receptor beta 2 in the developing chicken
Authors: Grommen, SVH ×
De Groef, Bert
Kühn, Eduard
Darras, Veerle #
Issue Date: Apr-2005
Publisher: New York Academy of Sciences
Series Title: Annals of the New York Academy of Sciences vol:1040 pages:328-331
Abstract: Thyroid hormones and their receptors (TRs) have critical functions in development and metabolism. In chicken, three TRs are known: TR alpha, TR beta 0, and TR beta 2. The latter was isolated from chicken eye, but its presence in other tissues has not yet been extensively investigated. We therefore developed a real-time PCR assay using a Taqman probe and primers based on the unique amino-terminal region of TR beta 2. We detected a strong TR beta 2 mRNA signal in the pituitary, confirmed with in situ hybridization, and in several other tissues. TR beta 2 mRNA was more abundant in the pituitary of newly hatched chicks than in 15-day-old embryos.
ISSN: 0077-8923
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
× corresponding author
# (joint) last author

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