Title: Transcript analysis of 250 novel yeast genes from chromosome XIV
Authors: Planta, RJ ×
Brown, AJP
Cadahia, JL
Cerdan, ME
De Jonge, M
Gent, ME
Hayes, A
Kolen, CPAM
Lombardia, LJ
Sefton, M
Oliver, SG
Thevelein, Johan
Tournu, H
Van Delft, YJ
Verbart, DJ
Winderickx, Joris #
Issue Date: Mar-1999
Publisher: John wiley & sons ltd
Series Title: Yeast vol:15 issue:4 pages:329-350
Abstract: The European Functional Analysis Network (EUROFAN) is systematically analysing the function of novel Saccharomyces cerevisiae genes revealed by genome sequencing. As part of this effort our consortium has performed a detailed transcript analysis for 250 novel ORFs on chromosome XIV. All transcripts were quantified by Northern analysis under three quasi-steady-state conditions (exponential growth on rich fermentative, rich non-fermentative, and minimal fermentative media) and eight transient conditions (glucose derepression, glucose upshift, stationary phase, nitrogen starvation, osmo-stress, heat-shock, and two control conditions). Transcripts were detected for 82% of the 250 ORFs, and only one ORF did not yield a transcript of the expected length (YNL285w). Transcripts ranged from low (62%), moderate (16%) to high abundance (2%) relative to the ACI mRNA, The levels of 73% of the 206 chromosome XIV transcripts detected fluctuated in response to the transient states tested. However, only a small number responded strongly to the transients: eight ORFs were induced upon glucose upshift: five were repressed by glucose; six were induced in response to nitrogen starvation three were induced in stationary phase; five were induced by osmo-stress; four were induced by heat-shock. These data provide useful clues about the general function of these ORFs and add to our understanding of gene regulation on a genome-wide basis. Copyright (C) 1999 John Wiley & Sons, Ltd.
ISSN: 0749-503X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Microbiology and Biotechnology Section - miscellaneous
Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
× corresponding author
# (joint) last author

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