Title: Characterization of an RFamide-related peptide orphan GPCR in C. elegans
Authors: Mertens, Inge
Vandingenen, A
Clynen, Elke
Nachman, RJ
De Loof, Arnold
Schoofs, Liliane # ×
Issue Date: 2005
Publisher: New York Academy of Sciences
Series Title: Annals of the New York academy of sciences vol:1040 pages:410-412
Abstract: We cloned and characterized an orphan FMRFamide-related peptide (FaRP) GPCR in Caenorhabditis elegans. We synthesized numerous structurally different FaRPs that were found in the C. elegans genome by bioinformatic analysis and used them to screen cells expressing the C26F1.6 receptor. Two peptides ending in M(orL)VRFamide elicited a calcium response in receptor-expressing mammalian Chinese hamster ovary cells. The response was dose-dependent and appeared to be very specific; that is, none of the other FaRPs were active, not even closely related peptides also ending in M(orL)VPF-amide, which are encoded by the same peptide precursor. Pharmacological profiling with a truncated series of the most active peptide revealed that the full peptide sequence is necessary for receptor activation.
ISSN: 0077-8923
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Department of Biology - miscellaneous
× corresponding author
# (joint) last author

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