Title: Dose-dependent induction of heat shock protein 70 synthesis in Raphidocelis subcapitata following exposure to different classes of environmental pollutants
Authors: Bierkens, J ×
Maes, Joachim
Vander Plaetse, F #
Issue Date: 1998
Publisher: Elsevier sci ltd
Series Title: Environmental pollution vol:101 issue:1 pages:91-97
Abstract: In this study an enzyme-linked immunosorbent assay (ELISA) was developed to detect the stress protein Hsp70 in the green alga Raphidocelis subcapitata. Using this ELISA, the response to a variety of pollutants, including ZnCl2, SeO2 (heavy metals), lindane (organochlorine pesticide), pentachlorophenol (PCP, chlorinated hydrocarbon insecticide and fungicide), carbaryl (carbamate pesticide) and sodium dodecyl sulphate (SDS; surfactant) was tested. Our results show that Hsp 70 is produced in a dose-dependent way in response to most chemicals investigated (except PCP) and at concentrations below the range of classical cytotoxicity testing (i.e. growth inhibition, lethality). Still, the potential to induce Hsp70 varied among the pollutants tested, the heavy metals ZnCl2 and SeO2 being the strongest inducers of Hsp70. Combined with the existing literature, these results indicate that Hsp70 in R. subcapitata is a sensitive biomarker for a wide range of environmental pollutants. (C) 1998 Elsevier Science Ltd. All rights reserved.
ISSN: 0269-7491
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory for Aquatic Ecology and Evolutionary Biology (-)
× corresponding author
# (joint) last author

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