Isolation, identification and localization of a second beetle antidiuretic peptide
Eigenheer, RA Wiehart, UM Nicolson, SW Schoofs, Liliane Schegg, KM Hull, JJ Schooley, DA # ×
Peptides vol:24 issue:1 pages:27-34
We isolated from head extracts of Tenebrio molitor a peptide that inhibits fluid secretion by the Malpighian tubules of this insect. This second antidiuretic factor, ADFb, like the previously published ADFa, works through cyclic GMP as a second messenger. It has primary structure Tyr-Asp-Asp-Gly-Ser-Tyr-Lys-Pro-His-Ile-Tyr-Gly-Phe-OH with an EC50 of approximately 240 pM in a fluid secretion assay. This peptide is now the second sequenced endogenous insect ADF which inhibits Malpighian tubule fluid secretion. Immunohistochemical techniques show that the peptide is localized in the brain; it appears to be produced mainly in two pairs of bilaterally symmetrical cells in the protocerebrum. (C) 2002 Elsevier Science Inc. All rights reserved.