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Title: Molecular cloning and characterization of a high DP fructan: fructan 1-fructosyl transferase from Viguiera discolor (Asteraceae) and its heterologous expression in Pichia pastoris
Authors: Van den Ende, Wim ×
Van Laere, André
Le Roy, Katrien
Vergauwen, Rudolf
Boogaerts, D
Figueiredo-Ribeiro, RCL
Machado de Carvalho, MA #
Issue Date: Dec-2005
Publisher: Blackwell publishing
Series Title: Physiologia plantarum vol:125 issue:4 pages:419-429
Abstract: Inulin- type fructans are stored in the tuberous roots of the Brazilian cerrado plant Viguiera discolor Baker ( Asteraceae). In Cynara scolymus ( artichoke) and Echinops ritro ( globe thistle), the fructans have a considerably higher degree of polymerization ( DP) than in Cichorium intybus ( chicory) and Helianthus tuberosus ( Jerusalem artichoke). It was shown before that the higher DP in some species can be attributed to the properties of their fructan: fructan 1- fructosyl transferases ( 1- FFTs; EC 2.4.1.100), enzymes responsible for chain elongation. Here, we describe the cloning of a high DP ( hDP) 1- FFT cDNA from V. discolor and its heterologous expression in Pichia pastoris. Starting from 1- kestose and Neosugar P ( a mixture of oligo- inulins from microbial origin) as substrates, the recombinant enzyme produces a typical hDP inulin profile in vitro, closely resembling the one observed in vivo. The enzyme shows no invertase activity and sucrose: sucrose 1- fructosyl transferase ( 1- SST; EC 2.4.1.99) activity in vitro. Pattern evolution during incubation suggests that inulins with DP >= 6 are much better substrates than sucrose or lower DP oligo- fructans. Because hDP inulin- type fructans show superior properties for specific food and non- food applications, the hDP 1- FFT gene from V. discolor has potential for the production of hDP inulin in vitro or in transgenic crops.
URI: 
ISSN: 0031-9317
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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