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Title: The expression in tobacco plants of Aedes aegypti Trypsin Modulating Oostatic Factor (Aea-TMOF) alters growth and development of the tobacco budworm, Heliothis virescens
Authors: Tortiglione, C ×
Fanti, P
Pennacchio, F
Malva, C
Breuer, Michael
De Loof, Arnold
Monti, LM
Tremblay, E
Rao, R #
Issue Date: 2002
Publisher: Kluwer academic publ
Series Title: Molecular breeding vol:9 issue:3 pages:159-169
Abstract: The production and characterisation of transgenic tobacco plants expressing a precursor of a regulatory peptide from Aedes aegypti (Trypsin Modulating and Oostatic Factor, Aea-TMOF) which interferes with the development of tobacco budworm larvae is described. Tobacco plants were transformed with a synthetic gene containing 6 TMOF units spaced by dibasic residues, Arg-Arg, as potential post-translational cleavage sites. Peptide extracts from transgenic plants had TMOF activity and inhibited in vitro the biosynthesis of serine proteases. This activity was consistently present in T1 plants and absent in control plants. Tobacco budworm larvae, fed with transgenic leaves showed a reduced growth rate compared to those fed with control plants. The low rather than acute toxicity of this low impact gene is discussed in the context of alternative integrated pest management strategies.
URI: 
ISSN: 1380-3743
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Department of Biology - miscellaneous
× corresponding author
# (joint) last author

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