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Title: The P5 Type ATPase Atp13A2, a Parkinson associated gene, coordinates key trafficking mechanisms involved in the maintenance of cancer cell proteostasis
Other Titles: P5 Type ATPase Atp13A2, een Parkinson geässocieerd gen, coördineert belangrijke communicatiemechanismen betrokken bij het onderhoud van kankercel proteostase
Authors: Demirsoy, Seyma
Issue Date: 16-Oct-2017
Abstract: ATP13A2 (also called PARK9), is a transmembrane endo-/lysosomal-associated P5-type transport ATPase. Loss-of-function mutations in ATP13A2 result in the Kufor-Rakeb Syndrome (KRS), a form of autosomal Parkinson’s disease (PD). In spite of a growing interest in ATP13A2, very little is known about its physiological role in stressed cells. Recent studies suggest that the N terminal domain of ATP13A2 may hold key regulatory functions, but their nature remain incompletely understood. To this end, we generated a set of melanoma and neuroblastoma cell lines stably overexpressing wild-type (WT), catalytically inactive (D508N) and N terminal mutants, or shRNA against ATP13A2. We found that under proteotoxic stress conditions, evoked by the proteasome inhibitor Borte, endo-/lysosomal associated full-length ATP13A2 WT, catalytically-inactive or N terminal fragment mutants, reduced the intracellular accumulation of ubiquitin-conjugated (Ub) proteins, independent of autophagic degradation. In contrast, ATP13A2 silencing increased the intracellular accumulation of Ub-proteins, a pattern also observed in patient-derived fibroblasts harbouring ATP13A2 loss-of function mutations. In treated cells, ATP13A2 evoked endocytic vesicle relocation and increased cargo export through nanovesicles. Expression of an ATP13A2 mutant abrogating PI(3,5)P2 binding or chemical inhibition of the PI(3,5)P2-generating enzyme PIKfyve, compromised vesicular trafficking/nanovesicles export and rescued intracellular accumulation of Ub-proteins in response to proteasomal inhibition. Hence, our study highlighted a novel activity-independent scaffolding role of ATP13A2 in trafficking/export of intracellular cargo in response to proteotoxic stress.
Publication status: accepted
KU Leuven publication type: TH
Appears in Collections:Laboratory of Cellular Transport Systems
Laboratory of Cell Death Research & Therapy

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