Author:

Keywords:

Science & Technology, Technology, Engineering, Biomedical, Materials Science, Biomaterials, Engineering, Materials Science, bioactive glass, anti-biofilm, bromide, sol-gel synthesis, bone infection, bone healing, IN-VITRO, ANTIBACTERIAL ACTIVITY, SURFACE-ROUGHNESS, BONE REPAIR, SILVER, NANOPARTICLES, PARTICLES, SCAFFOLDS, GROWTH, COPPER, Bacteria, Bacterial Physiological Phenomena, Biofilms, Bromides, Glass, Mouth, 03 Chemical Sciences, 06 Biological Sciences, 09 Engineering, 31 Biological sciences, 34 Chemical sciences, 40 Engineering

Abstract:

Bioactive glass is an attractive biomaterial that has shown excellent osteogenic and angiogenic effects for oral bone repairing procedures. However, anti-biofilm potential related to such biomaterial has not been completely validated, mainly against multispecies biofilms involved in early tissue infections. The aim of the present study was to evaluate the anti-biofilm effect of 58S bioactive glass embedding calcium bromide compounds at different concentrations. Bioactive glass containing 0, 5, or 10wt% CaBr2 was synthesized by alkali sol-gel method and then characterized by physco-chemical and scanning electron microscopy (SEM). Then, samples were tested by microbiological assays using optical density, real time q-PCR, and SEM. Bioactive glass particles showed accurate chemical composition and an angular shape with a bimodal size distribution ranging from 0.6 to 110 μm. The mean particle size was around 29 μm. A significant anti-biofilm effect was recorded for 5wt% CaBr2-doped bioactive glass against S. mitis, V. parvula, P. gingivais, S. gordoni, A. viscosus, F, nucleatum, P. gingivais. F. nucleatum and P. gingivalis. Such species are involved in the biofilm structure related to infections on hard and soft tissues in the oral cavity. The incorporation of calcium bromide into bioactive glass can be a strategy to enhance the anti-biofilm potential of bioactive glasses for bone healing and infection treatment. Key words: Bioactive glass, anti-biofilm, bromide, sol-gel synthesis, bone infection, bone healing