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Title: Split-BioID: a proximity-biotinylation assay for dimerization-dependent protein interactions
Authors: De Munter, Sofie ×
Goernemann, Janina
Derua, Rita
Lesage, Bart
Qian, Junbin
Heroes, Ewald
Waelkens, Etienne
Van Eynde, Aleyde
Beullens, Monique
Bollen, Mathieu #
Issue Date: Jan-2017
Publisher: Elsevier on behalf of the Federation of European Biochemical Societies
Series Title: FEBS Letters vol:591 issue:2 pages:415-424
Article number: 10.1002/1873-3468.12548
Abstract: The biotin-identification (BioID) protocol uses a mutant of the biotin ligase BirA (BirA*) fused to a protein-of-interest to biotinylate proximate proteins in intact cells. Here, we show that two inactive halves of BirA* separately fused to a catalytic and regulatory subunit of protein phosphatase PP1 reconstitute a functional BirA* enzyme upon heterodimerization of the phosphatase subunits. We also demonstrate that this BirA*-fragment complementation approach, termed split-BioID, can be used to screen for substrates and other protein interactors of PP1 holoenzymes. Split-BioID is a novel and versatile tool for the identification of (transient) interactors of protein dimers. This article is protected by copyright. All rights reserved.
URI: 
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Cellular and Molecular Medicine - miscellaneous
Studiegebied Handelswetenschappen & Bedrijfskunde - VIVES Zuid
Laboratory of Protein Phosphorylation and Proteomics
Laboratory of Biosignaling & Therapeutics
× corresponding author
# (joint) last author

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