Title: Array painting using microdissected chromosomes to map chromosomal breakpoints
Authors: Backx, Liesbeth ×
Van Esch, Hilde
Melotte, C
Kosyakova, N
Starke, H
Fryns, Jean-Pierre
Liehr, T
Vermeesch, Joris #
Issue Date: 2007
Publisher: S. Karger
Series Title: Cytogenetic and Genome Research vol:116 issue:3 pages:158-166
Abstract: Molecular characterization of breakpoints of chromosomal rearrangements is a successful strategy for the identification of candidate disease genes. Mapping translocation breakpoints and rearranged chromosomal boundaries is labor intensive and/or time consuming. Here, we present a novel and rapid procedure to map such chromosomal breakpoints by hybridizing amplified microdissection derived DNA of aberrant chromosomes to arrays containing genomic clones. We illustrate the potential of the technique by molecularly delineating the breakpoints in five small supernumerary marker chromosomes (sSMC) and mapping the breakpoints of five different chromosomal translocations.
ISSN: 1424-8581
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Clinical Genetics Section (-)
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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